RNA

Part:BBa_K1963002:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-07)


A template for expression of sRNA fusions to the E. coli micC hairpin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

The sequence is RFC[10] compliant.


Source

This was designed de novo and synthesised as a gBlock by IDT for the Dundee_Schools iGEM team in 2016.

References

Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105